Meloidogyne arenaria candidate effector MaMsp4 interacts with maize (Zea mays L.) proteins involved in host defense response and cell wall modifications
نویسندگان
چکیده
Abstract Background and aims Meloidogyne arenaria is an economically important root-knot nematode species. Successful plant infection by facilitated parasite effectors. This study aimed to characterize a candidate M. effector, indicate its molecular partners from maize, analyze role during infection. Material methods At first, we performed EST database mining find effector protein . The expression of coding gene in developmental stages was assessed using digital droplet PCR. Candidate were determined yeast two-hybrid screening maize cDNA library interactions confirmed co-immunoprecipitation after co-expression Nicotiana benthamiana GFP-fused localization N. leaves observed under confocal microscope. Then, level genes encoding interacting proteins measured. Results MaMsp4 evaluated as the highest stage J2. indicated, with beta-galactosidase 11, pectinesterase, S-adenosyl methionine decarboxylase 2, ethanolamine-phosphate cytidylyltransferase confirmed, all fused GFP detected apoplast and/or cytoplasm. Genes 11 playing cell wall modifications, overexpressed at 24 hpi followed down-regulation 7 dpi, while 2 cytidylyltransferase, involved defense response, suppressed without preceding up-regulation. Conclusions We have found that interacts modifications mechanisms related polyamines biosynthesis.
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ژورنال
عنوان ژورنال: Plant and Soil
سال: 2023
ISSN: ['0032-079X', '1573-5036']
DOI: https://doi.org/10.1007/s11104-023-06130-3